![]() Depending on the oligo properties, a rating is assigned. The design results are reported in a ranked order, giving you a list of alternates to choose from. With any of these chemistries, you could evaluate a pre-designed/published primers or TaqMan® probes. Using Beacon Designer™, you can design suitable control primers and probes by designing oligos for the methylated and the unmethylated strands, and for the untreated DNA sequence. MethyLight is a high throughput assay used to discriminate between methylated and unmethylated DNA. To study DNA methylation, Beacon Designer™ helps design primers and TaqMan® probes for MethyLight assays. You can also choose to design the increasingly popular Locked Nucleic Acid (LNA™) substituted TaqMan® probes, which are more stable than standard TaqMan® probes. Use Beacon Designer™ for optimal TaqMan® probes that help you study the differential gene expression or study SNP (single nucleotide polymorphisms) using TaqMan® SNP genotyping assays. Dual Labeled Probe Designīeacon Designer™ currently supports four probe based chemistries: Pre-designed or published primers can also be analyzed. The HRMA primers designed can be BLAST searched against nucleotide databases at NCBI to check their specificity. The primers are designed avoiding template secondary structures, assuring efficient primer extension. High Resolution Melting Analysis primers are designed flanking a mutation of interest to generate the shortest possible amplicons with detectable melting temperature variation. The program employs proprietary algorithms that enables designing the best primers for detecting mutations. High Resolution Melting Analysis (HRMA) is a more cost-effective method than probe-based genotyping analysis. HRMA Assay Designīeacon Designer™ offers a comprehensive solution for mutation detection. These primers designed across exon intron junctions help selective amplification of cDNA from gDNA. In addition to locating the primer anywhere on the gene of interest, Beacon Designer can design primers across exon-exon or exon-intron junctions. You can even choose your own default design parameters for your specific research needs. You have complete control over the design parameters or you can use the default values, chosen after considerable research and communication with experts. Beacon Designer™ designs SYBR® Green PCR primers and helps you evaluate pre-designed SYBR® Green PCR primer sets. SYBR® Green is perhaps the most commonly used real time PCR chemistry. Design Options Available SYBR® Green Assay Design The regions that exhibit significant cross homologies and template structures are avoided automatically during the design. ![]() The results of both these searches are used while designing primers and probes. You can BLAST search sequences and search for template structures from within the program. Specific and Efficient Design: How Beacon Designer™ Makes it Possible? Micro, Biochem, Immuno Morehouse School of Medicine, Atlanta, GA And it has saved money because I don't have to purchase several expensive labeled probes to test which one will work the best." - Dr. ![]() "We love your program - it has saved time in that we don't have to use 3 or 4 different programs to determine how well the probes we have designed will bind to other sequences. Beacon Designer™ is a flexible solution to your real time primer and probe design needs and pays for itself many times over. It saves the time and the money involved in failed experiments. It is used by molecular biologists worldwide to design successful real time PCR assays. Beacon Designer™ Automate Your Real Time PCR Primer and Probe Designīeacon Designer™ automates the design of real time primers and probes.
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